| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Peptides | Subsequently, DMSO-conditioned medium or YKL-5-124-conditioned medium were collected and added to above single- cell suspension in a 96-well U-bottom plate in the presence of Ova257–264 peptide (10 mg/ml, GenScript, Cat#RP10611) for 4 days as previously described | Get A Quote |
Cyclin-dependent kinase 7 (CDK7) is a central regulator of the cell cycle and gene transcription. However, little is known about its impact on genomic instability and cancer immunity. Using a selective CDK7 inhibitor, YKL-5-124, we demonstrated that CDK7 inhibition predominately disrupts cell-cycle progression and induces DNA replication stress and genome instability in small cell lung cancer (SCLC) while simultaneously triggering immune-response signaling. These tumor-intrinsic events provoke a robust immune surveillance program elicited by T cells, which is further enhanced by the addition of immune-checkpoint blockade. Combining YKL-5-124 with anti-PD-1 offers significant survival benefit in multiple highly... More
