| Products/Services Used | Details | Operation |
|---|---|---|
| Bacterial Expression System | The molecular signals used to induce low abundance transcripts were: (1) the phytotoxin coronatine (#C8115, Sigma Aldrich), a jasmonoyl-L-isoleucine (JA-Ile) molecular mimicry, deployed by several strains of Pseudomonas syringae (Bender et al.1999) to overcome plant defenses (Melotto and Kunkel, 2013); (2) flg22 (#RP19986, GenScript), a MAMP (Microbe Associated Molecular Pattern) with a conserved segment of 22 amino acids present in the bacterial motor protein flagellin. | Get A Quote |
Yeast-two-hybrid (Y2H) cDNA library screening is a valuable tool to uncover protein-protein interactions and represents a widely used method to investigate protein function. However， low transcript representation in cDNA libraries limits the depth of the screening. We have developed a Y2H library with cDNA made from Arabidopsis leaves exposed to several stressors as well as untreated leaves. The library was built using pooled mRNA extracted from plants challenged with plant and human bacterial pathogens， the flg22 elicitor， the phytotoxin coronatine， and several hormones associated with environmental stress responses. The purpose of such a library is to maximize the discovery of protein-protein interact... More
